The RNA Transcript, June 20, 2022 |
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Samantha Grudzien is a Neuroscience graduate student in the Peter Todd Lab. She is interested in how genetic variations drive neurodegenerative diseases. She hopes her vivo mouse, and complementary in vitro patient-derived data, will provide rigorous evidence that will lead to clinical, RNA-based therapies for Fragile X-associated tremor/ataxia syndrome (FXTAS), the neurodegenerative disease she studies.
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Harvard Medical School Initiative for RNA Medicine |
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CRISPR-powered (long noncoding) RNA therapeutics for oncology
Monday, June 20, 2022
3:00 - 4:00 PM
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U-M Oral Dissertation Defense |
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Statistical Methods and Computational Tools for High-throughput Genomics Data
Tuesday, June 21, 2022
9:00 - 11:00 AM
M1122, SPH2
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RNA Therapeutics Institute (RTI) |
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4th Annual RNATx Symposium
Virtual event with talks, posters and more..
June 22-24, 2022
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RNA Collaborative Seminar Series |
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----Wednesday, June 29, 2022
----10:00 am - 11:00 am
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RNA-host protein interaction analyses reveal additional proteins involved in SARS-CoV-2 RNA biology
Dissecting the role of Piwil2 (Mili) in neural stem cell fate
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Repeat-Associated Non-AUG Translation of CGG Repeats in Native Neuronal Function and Disease
Monday, June 27, 2022
1:00 - 2:00 PM
Kahn, BSRB
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Rustbelt RNA Meeting (RRM) |
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The Rustbelt RNA Meeting (RRM) is a regional scientific meeting that gathers scientists from throughout the Midwestern and Mid-Atlantic United States to discuss RNA-related biomedical research.
SAVE THE DATE!
October 14–15, 2022
CWRU & Cleveland Clinic, Cleveland Ohio
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Catalyzing translational research efforts by supporting preclinical and commercial clinical development |
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Innovation Partnerships at the University of Michigan manages Great Lakes Discovery, a ten-year collaboration with Deerfield Management Company, L.P to fund U-M drug discovery from early-stage research to clinical development.
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Deerfield has committed up to $130 million over the next decade to invest in biomedical research at U-M with the aim of developing potentially life-saving drugs and disease treatments.
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Projects selected by Great Lakes Discovery will be provided funding and operational support through a development plan aimed at achieving Investigational New Drug (IND) readiness.
GET STARTED WITH A CONSULTATION There is no open Request for Proposal (RFP) - the first step for an interested researcher is to schedule a consultation with the program’s Scientific Collaboration Manager Dr. Seohee You (shyou@umich.edu).
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Our members' publications are available through Altmetrics. Queries are currently available: CRISPR, microRNA, molecule, RNA, RNA therapeutics, transcriptome, and translation. Below are recent highlights.
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Highlights
- Long noncoding RNAs (lncRNAs) are tissue- and context-specific modulators of immune cell development, differentiation, activation, and function.
- lncRNA molecular mechanisms are diverse and challenging to predict.
- lncRNAs impact on the outcomes of protective and pathogenic immune responses in a variety of diseases.
- lncRNA genes outnumber protein-coding genes, but comparatively few lncRNAs have been functionally or mechanistically characterized.
- Further unlocking the role of lncRNAs in immunity may lead to improved outcomes for diseases that are influenced by protective and pathogenic immune responses.
Despite an ever-increasing appreciation of how protein-coding genes shape immune responses, the molecular underpinnings of immune regulation remain incompletely understood. This incomplete picture impedes the development of more precise therapeutics and diagnostics for immune-mediated diseases. Long noncoding RNAs (lncRNAs) are versatile cell- and context-specific regulators of gene expression and cellular function. The number of lncRNA genes rivals that of protein-coding genes; however, comparatively little is known about their function. Even though the functions of most lncRNA genes are unknown, multiple lncRNAs have recently emerged as important immune regulators. Therefore, further unlocking the role of lncRNAs in the mammalian immune system coupled with their tissue-specific expression might lead to more precise therapeutics and diagnostics for immune disorders in general.
Keywords: long noncoding RNA, immunity, autoimmunity, inflammatory immune disorders, alloimmunity, antitumor immunity
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Tsoi, L.C., Xing, X., Kahlenberg, J. M., Scott, V. E., & Gudjonsson, J. (2022). Noninvasive Tape-Stripping with High-Resolution RNA Profiling Effectively Captures a Preinflammatory State in Nonlesional Psoriatic Skin. Journal of Investigative Dermatology, 142(6), 1587–1596. https://doi.org/10.1016/j.jid.2021.09.038
Tape stripping is a minimally invasive, nonscarring method that can be utilized to assess gene expression in the skin but is infrequently used given technical constraints. By comparing different tape stripping technologies and full-thickness skin biopsy results of lesional and nonlesional psoriatic skin from the same patients, we demonstrate that tape stripping with optimized high-resolution transcriptomic profiling can be used to effectively assess and characterize inflammatory responses in the skin. Upon comparison with single-cell RNA-sequencing data from psoriatic full-thickness skin biopsies, we illustrate that tape-stripping efficiently captures the transcriptome of the upper layers of the epidermis with sufficient resolution to assess the molecular components of the feed-forward immune amplification pathway in psoriasis. Notably, nonlesional psoriatic skin sampled by tape stripping demonstrates activated, proinflammatory changes when compared to healthy control skin, suggesting a prepsoriatic state, which is not captured on full-thickness skin biopsy transcriptome profiling. This work illustrates an approach to assess inflammatory response in the epidermis by combining noninvasive sampling with high throughput RNA-sequencing, providing a foundation for biomarker discoveries and mechanism of action studies for inflammatory skin conditions.
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