The RNA Transcript, July 11, 2022 |
|
The U-M Center for RNA Biomedicine is looking for U-M Graduate Students and Postdocs to join its RNA Student & Postdoc Council for the 2022–23 academic year.
The objective of this council is to work collaboratively across disciplines, build a scientific community, and generate ideas and activities that advance RNA research and education across the University of Michigan.
This is a great opportunity to develop and implement ideas that enhance your curriculum and experience at U-M. The Council is also an excellent platform to further engage with peers, mentors, and faculty from the RNA research community. To join, email Martina Jerant by Friday, July 29 th
|
|
RNA Collaborative Seminar Series |
|
----Wednesday, July 13, 2022
----10:00 am - 11:00 am
|
|
FUBP1 is a new core component of 3' splice site recognition that facilitates the splicing of long introns
High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance
|
|
|
|
|
The RiboClub Society has set aside a limited amount of funds for fellowships to support students and postdoctoral fellows who otherwise would not be able to attend the meeting for financial reasons.
Sep 18 – 22, 2022
Quebec, Canada
|
|
U-M Bioinnovations in Brain Cancer |
|
|
|
The Inaugural Brain Cancer Symposium brings together experts from around the world in a weekend full of talks, posters, and social events.
Sep 30 – Oct 1, 2022
Ann Arbor, MI
|
|
Midwest Oligonucleotide Therapeutics Symposium |
|
|
|
The Midwest Symposium on Oligonucleotide Therapeutics is a grassroots meeting in Indianapolis supported by an Oligonucleotide Therapeutics Society (OTS) Local Delivery Grant.
Oct 8 – 9, 2022
Indianapolis, IN
|
|
The RNA Society of North Carolina |
|
|
|
The symposium will feature world-class speakers, talks from submitted abstracts, and many great opportunities for networking. Travel awards will be given out on a rolling basis.
Oct 13–14, 2022
North Carolina Biotechnology Center
|
|
Rustbelt RNA Meeting (RRM) |
|
|
|
The Rustbelt RNA Meeting (RRM) is a regional scientific meeting that gathers scientists from throughout the Midwestern and Mid-Atlantic United States to discuss RNA-related biomedical research.
Oct 14–15, 2022
Cleveland, OH
|
|
|
|
Our members' publications are available through Altmetrics. Queries are currently available: CRISPR, microRNA, molecule, RNA, RNA therapeutics, transcriptome, and translation. Below are recent highlights.
|
|
Highlights
- Long noncoding RNAs (lncRNAs) are tissue- and context-specific modulators of immune cell development, differentiation, activation, and function.
- lncRNA molecular mechanisms are diverse and challenging to predict.
- lncRNAs impact on the outcomes of protective and pathogenic immune responses in a variety of diseases.
- lncRNA genes outnumber protein-coding genes, but comparatively few lncRNAs have been functionally or mechanistically characterized.
- Further unlocking the role of lncRNAs in immunity may lead to improved outcomes for diseases that are influenced by protective and pathogenic immune responses.
Despite an ever-increasing appreciation of how protein-coding genes shape immune responses, the molecular underpinnings of immune regulation remain incompletely understood. This incomplete picture impedes the development of more precise therapeutics and diagnostics for immune-mediated diseases. Long noncoding RNAs (lncRNAs) are versatile cell- and context-specific regulators of gene expression and cellular function. The number of lncRNA genes rivals that of protein-coding genes; however, comparatively little is known about their function. Even though the functions of most lncRNA genes are unknown, multiple lncRNAs have recently emerged as important immune regulators. Therefore, further unlocking the role of lncRNAs in the mammalian immune system coupled with their tissue-specific expression might lead to more precise therapeutics and diagnostics for immune disorders in general.
Keywords: long noncoding RNA, immunity, autoimmunity, inflammatory immune disorders, alloimmunity, antitumor immunity
|
|
Tsoi, L.C., Xing, X., Kahlenberg, J. M., Scott, V. E., & Gudjonsson, J. (2022). Noninvasive Tape-Stripping with High-Resolution RNA Profiling Effectively Captures a Preinflammatory State in Nonlesional Psoriatic Skin. Journal of Investigative Dermatology, 142(6), 1587–1596. https://doi.org/10.1016/j.jid.2021.09.038
Tape stripping is a minimally invasive, nonscarring method that can be utilized to assess gene expression in the skin but is infrequently used given technical constraints. By comparing different tape stripping technologies and full-thickness skin biopsy results of lesional and nonlesional psoriatic skin from the same patients, we demonstrate that tape stripping with optimized high-resolution transcriptomic profiling can be used to effectively assess and characterize inflammatory responses in the skin. Upon comparison with single-cell RNA-sequencing data from psoriatic full-thickness skin biopsies, we illustrate that tape-stripping efficiently captures the transcriptome of the upper layers of the epidermis with sufficient resolution to assess the molecular components of the feed-forward immune amplification pathway in psoriasis. Notably, nonlesional psoriatic skin sampled by tape stripping demonstrates activated, proinflammatory changes when compared to healthy control skin, suggesting a prepsoriatic state, which is not captured on full-thickness skin biopsy transcriptome profiling. This work illustrates an approach to assess inflammatory response in the epidermis by combining noninvasive sampling with high throughput RNA-sequencing, providing a foundation for biomarker discoveries and mechanism of action studies for inflammatory skin conditions.
|
|
|
|
|
|
|
