A Rogel Cancer Center Shared Resources Newsletter |
|
News and information about Shared Resources for Rogel Cancer Center members
|
|
A MESSAGE FROM DR. KELLER |
|
Dear Rogel Cancer Center Members,
Welcome to another issue of Instrumental.
I would like to highlight some exciting changes happening in the future of Rogel Cancer Center Shared Resources. We are now working on several new developing shared resources including the Liquid Biopsy Shared Resource, which will be directed by Sunitha Nagrath, Ph.D., Simpa Salami, M.D., M.P.H., and Aaron Udager, M.D., Ph.D.; and joining this new offering will be the Epigenomics and Epigenetics Shared Resource, directed by Dana Dolinoy, Ph.D. and managed by Claudia Lalancette, Ph.D. The Liquid Biopsy Shared Resource is not ready to offer services yet but is working hard towards getting some initial services up and running. The Epigenomics and Epigenetics Shared Resource is an affiliation with the BRCF Epigenomics core and is actively taking samples.
In other news, the Rogel Single Cell Analysis Shared Resource has merged with the BRCF Advanced Genomics Core and has been renamed the Single Cell and Spatial Analysis Shared Resource. This shared resource is co-directed by Tom Wilson, M.D., Ph.D., and Evan Keller, D.V.M., Ph.D. and managed by Olivia Koues, Ph.D. It now offers full services from cell preparation through single cell / nuclei and spatial analytic procedures and an initial bioinformatic analysis.
Finally, I would like to acknowledge all the efforts and expertise provided by our shared resources directors, managers, and staff. Above and beyond the fancy equipment and procedures, these dedicated teams are what make the shared resources possible and move the Rogel Cancer Center research machine forward.
Take care and as always, please feel free to reach out to me with any questions or concerns.
Thanks for your time,
Evan T. Keller, D.V.M., Ph.D.
Associate Director for Shared Resources
Rogel Cancer Center
|
|
Shared Resources Training Seminar
Positron emission tomography: molecular imaging in the age of personalized medicine presented by Peter J. H. Scott, Ph.D., co-director, Preclinical Molecular Imaging Shared Resource
|
|
Imaging mass cytometry (IMC)
IMC is a state-of-the-art technology available at the CyTOF core of the Rogel Cancer Center Immune Monitoring Shared Resources that allows simultaneous imaging of 40 parameters at 1 µm resolution. IMC uses antibodies conjugated to heavy metals to detect markers in a manner analogous to immuno-histochemistry or immunofluorescence microscopy.
The CyTOF core offers two IMC antibody panels for cancer research:
- The Human Cancer Panel focuses on characterizing cancer cells and includes markers for stemness, differentiation, and clinical subtypes.
- The Human Immune Panel is used to interrogate the tumor immune microenvironment, which has recently emerged as an important area in cancer research.
Furthermore, the CyTOF core offers custom antibody conjugation services so users can include additional antibodies pertinent to their research in IMC experiments. The CyTOF core also offers services for the suspension CyTOF which can detect 40 colors in single cell suspensions.
For more information about IMC and other services offered by the CyTOF core, please visit the CyTOF core website. |
|
Annual Shared Resources Survey Results
The 2021 survey was sent to 317 members of whom, 82 responded for an overall response rate of 25.9%. Members rated our SRs favorably in Overall Performance with a user satisfaction rate of 87%. Some issues were identified that we are working to improve.
The SR survey also provides members with an opportunity to identify future services and technologies that would benefit their research. A few of the requested technologies are already supported by Rogel SRs:
REQUEST: Automated multiplex IHC System
A Vectra Polaris mfIHC is available within our Tissue and Molecular Pathology SR. The capabilities of this system were discussed in conjunction with the March SR Training Seminar. A recording of this, and other SR Training Seminars, can be found here.
REQUEST: Light sheet microscopy and live-cell super resolution microscopy, in vivo 2-photon microscopy
The BRCF Microscopy core hosts these and other state-of-the-art microscopy instruments. A full list of equipment is available here.
REQUEST: Spatial transcriptomics
The Single Cell and Spatial Analysis SR has partnered with the Advanced Genomics Core and the Single Cell Spatial Analysis Program to support spatial transcriptomics on both the 10X Genomics Visium and Nanostring GeoMX platforms. Additionally, a Single Cell Spatial Analysis Monthly Seminar Series is held the third Monday of each month as advertised on the Rogel Events Calendar.
REQUEST: Webinars for bioinformatics
Cancer Data Science hosts regular webinars. View their website for upcoming events and for already recorded sessions. |
|
Thomas Saunders, Ph.D., Director of the Transgenic Animal Model Core retires June 30
Dr. Saunders has been an essential member of the University of Michigan research community for over 30 years and has been with the BRCF Transgenic Animal Model Core since its founding in 1989. As the Core Director, Dr. Saunders focused his career on generating new mouse and rat models with genetic modifications that can serve as models of human genetic disease. Under his leadership, the Transgenic Core has produced well over 30,000 transgenic models, including the introduction of a CRISPR/Cas9 pipeline in 2013 that is continuously updated with the latest CRISPR/Cas9 technology.
|
|
|
We want to hear your feedback!
|
|
|
|
|
|
|
