RPMI Agar (RPMI1640, MOPS, L-glutamine, 2% Glucose) is intended for the AFST (antifungal susceptibility testing) and is recommended for usage with Antifungal MTS™ (MIC Test Strip).
Pick 4-5 colonies from 24-hours old cultures and suspend in saline solution or sterile water in order to obtain a 0.5 McFarland cell density.
Introduce a sterile swab into the suspension and inoculate the RPMI Agar plate.
Allow the plate to dry, position the antifungal strip onto the agar surface with the maximum concentration nearest the periphery of the plate.
Incubate at 35 °C for 24-48 hours.
Read the M.I.C. value where the edge of the inhibition ellipse intersects the strip (intersection between two scale segments should be round up to the higher value).
Available ready-to-use formats:
Ref. 11509
90 mm agar plate
20/pack
Ref. 10233
140 mm agar plate
10/pack
10-25°C storage temperature
180 days shelf life
