The RNA Transcript, June 27, 2022 |
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Nico Gomez is an MSTP student in the Sami Barmada Lab. He is primarily interested in exploring how the regulation of nucleic acid, the primary bearer of durable biological information across all living things, can be used to understand and treat disorders of the brain. In the past 5 years Nico has been focused on elucidating the mechanism(s) of action of an experimental therapeutic target for amyotrophic lateral sclerosis (ALS). Over this time, he's encountered both expected, and perhaps more importantly, unexpected results that he hopes will help guide resercherss in the design of more targeted and effective therapies for ALS.
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Repeat-Associated Non-AUG Translation of CGG Repeats in Native Neuronal Function and Disease
Monday, June 27, 2022
1:00 - 2:00 PM
Kahn, BSRB
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Harvard Initiative for RNA Medicine |
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Recurrent splice-site mutations affect key lymphoma genes
Tuesday, June 28, 2022
2:00 - 3:00 PM
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Mechanisms of Nutrient Sensing and Gene Regulation by the E. Coli Leucine-Responsive Regulatory Protein, a Global Feast-Famine Transcriptional Regulator
Wednesday, June 29, 2022
9:00 - 10:00 AM
North Lecture Hall, Med Sci II (and Zoom, password BIOCHEM) |
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Uncovering the Biological Basis of Racial Disparities in Breast Cancer: Interactions Between Breast Stem Cell Biology and Environmental Stressors
Wednesday, June 29, 2022
9:30 - 10:30 AM
Palmer Commons, Forum Hall
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RNA Collaborative Seminar Series |
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----Wednesday, June 29, 2022
----10:00 am - 11:00 am
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RNA-host protein interaction analyses reveal additional proteins involved in SARS-CoV-2 RNA biology
Dissecting the role of Piwil2 (Mili) in neural stem cell fate
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Mechanisms of Phenotypic Evolution at Different Levels of Biological Organization: From Molecules to Organisms
Wednesday, June 29, 2022
12:00 - 1:00 PM
1010 BSB
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The RiboClub Society has set aside a limited amount of funds for fellowships to support students and postdoctoral fellows who otherwise would not be able to attend the meeting for financial reasons. Abstracts Due June 30
September 18 – 22, 2022
Quebec, Canada
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U-M Bioinnovations in Brain Cancer |
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Sep 30 – Oct 1, 2022
Ann Arbor, MI
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Midwest Oligonucleotide Therapeutics Symposium |
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The Midwest Symposium on Oligonucleotide Therapeutics is a grassroots meeting in Indianapolis supported by an Oligonucleotide Therapeutics Society (OTS) Local Delivery Grant.
October 8 – 9, 2022
Indianapolis
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The RNA Society of North Carolina |
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The symposium will feature world-class speakers, talks from submitted abstracts, and many great opportunities for networking. Travel awards will be given out on a rolling basis, so REGISTER and submit your abstracts soon!
October 13–14, 2022
North Carolina Biotechnology Center
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Our members' publications are available through Altmetrics. Queries are currently available: CRISPR, microRNA, molecule, RNA, RNA therapeutics, transcriptome, and translation. Below are recent highlights.
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Highlights
- Long noncoding RNAs (lncRNAs) are tissue- and context-specific modulators of immune cell development, differentiation, activation, and function.
- lncRNA molecular mechanisms are diverse and challenging to predict.
- lncRNAs impact on the outcomes of protective and pathogenic immune responses in a variety of diseases.
- lncRNA genes outnumber protein-coding genes, but comparatively few lncRNAs have been functionally or mechanistically characterized.
- Further unlocking the role of lncRNAs in immunity may lead to improved outcomes for diseases that are influenced by protective and pathogenic immune responses.
Despite an ever-increasing appreciation of how protein-coding genes shape immune responses, the molecular underpinnings of immune regulation remain incompletely understood. This incomplete picture impedes the development of more precise therapeutics and diagnostics for immune-mediated diseases. Long noncoding RNAs (lncRNAs) are versatile cell- and context-specific regulators of gene expression and cellular function. The number of lncRNA genes rivals that of protein-coding genes; however, comparatively little is known about their function. Even though the functions of most lncRNA genes are unknown, multiple lncRNAs have recently emerged as important immune regulators. Therefore, further unlocking the role of lncRNAs in the mammalian immune system coupled with their tissue-specific expression might lead to more precise therapeutics and diagnostics for immune disorders in general.
Keywords: long noncoding RNA, immunity, autoimmunity, inflammatory immune disorders, alloimmunity, antitumor immunity
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Tsoi, L.C., Xing, X., Kahlenberg, J. M., Scott, V. E., & Gudjonsson, J. (2022). Noninvasive Tape-Stripping with High-Resolution RNA Profiling Effectively Captures a Preinflammatory State in Nonlesional Psoriatic Skin. Journal of Investigative Dermatology, 142(6), 1587–1596. https://doi.org/10.1016/j.jid.2021.09.038
Tape stripping is a minimally invasive, nonscarring method that can be utilized to assess gene expression in the skin but is infrequently used given technical constraints. By comparing different tape stripping technologies and full-thickness skin biopsy results of lesional and nonlesional psoriatic skin from the same patients, we demonstrate that tape stripping with optimized high-resolution transcriptomic profiling can be used to effectively assess and characterize inflammatory responses in the skin. Upon comparison with single-cell RNA-sequencing data from psoriatic full-thickness skin biopsies, we illustrate that tape-stripping efficiently captures the transcriptome of the upper layers of the epidermis with sufficient resolution to assess the molecular components of the feed-forward immune amplification pathway in psoriasis. Notably, nonlesional psoriatic skin sampled by tape stripping demonstrates activated, proinflammatory changes when compared to healthy control skin, suggesting a prepsoriatic state, which is not captured on full-thickness skin biopsy transcriptome profiling. This work illustrates an approach to assess inflammatory response in the epidermis by combining noninvasive sampling with high throughput RNA-sequencing, providing a foundation for biomarker discoveries and mechanism of action studies for inflammatory skin conditions.
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