Actin Up: Detecting a Key Cytoskeleton Protein

In HeLa cells, actin expression (green) was detected using a mouse anti-actin antibody (M-1646-100), revealing the submembranous cytoskeleton and stress fibers. Simultaneously, a chicken antibody to vimentin (C-1409-50; red) highlighted a distinct 10 nm filament cytoskeletal network.

Western blot analysis of tissue and cell lysates with mouse anti-actin antibody (M-1646-100; red) showed actin expression in various samples. Simultaneously, chicken antibody against UCHL1 (C-1406-50; green) confirmed neuronal lineage marker expression.

Flow Cytometry

Specific staining of Actin in human neuroblastoma SH-SY5Y cells was conducted via Flow Cytometry using actin antibody (M-1646-100). A negative control using Non-specific Control IgG, clone X63 (M-1249-200, black dashed) was included. Data and results were generated using the Orflo MoxiflowTM instrument and protocols.

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