Chlorolab 2+: The liquid-phase oxygen electrode system for advanced photosynthesis & respiration studies - From chloroplast extractions to mitochondrial suspensions!

O 2 evolution or uptake measurements across a wide range of samples!

The Chlorolab 2+ is an advanced system for the study of respiration and photosynthesis from liquid samples under automated illumination from a white, red or blue LED light source. The system automates the acquisition of oxygen evolution/uptake rates over user-defined light intensities to determine the apparent quantum yield. Advantages of the Chlorolab 2+ include:

PC-operated USB Oxylab+ electrode control unit DW2/2 advanced electrode chamber with 4 optical ports & integral S1 oxygen electrode disc LED1/W white LED light source (up to 2,000 µmol m-2 s-1) with automated control via user-defined PFD light tables Suitable for liquid-phase samples between 0.2ml – 2.5ml (min. 1.5ml if illuminated) with 0% – 100% oxygen concentration 24-bit high-resolution measurement of oxygen signals Integral systems for measurement of pH & other ion-selective electrode (ISE) signals with 16-bit resolution Onboard LCD readings of oxygen, auxiliary & ISE signals 2-channel capability via purchase of additional systems Quantitherm PAR/Temp sensor for light source calibration OxyTrace+ Windows® software for data acquisition, hardware control & data analysis Real time 0V – 4.5V analogue output of oxygen signal.

The individual components of the Chlorolab 2+ System include:

S1 Oxygen Electrode Disc When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration. DW2/2 Electrode Chamber The DW2/2 electrode chamber has been Developed specifically to allow simultaneous spectroscopic and oxygen flux measurements. The black acetal water jacket allows for dark adaptation of samples or oxygen measurement in complete darkness.

Oxylab+ Control Unit

Oxylab+ provides a convenient yet powerful tool for measurements of oxygen evolution or uptake across a broad range of liquid-phase samples from chloroplast extractions to mitochondrial suspensions with oxygen concentrations up to 100%. 24-bit resolution allows detection of minute changes in oxygen tension without needing to apply instrument gain, resulting in beautiful noise-free traces even when zoomed in on areas of interest. Integral electronics provide control over an LED light source with automatic intensity changes handled by user-defined PFD light tables in software.

The system allows real-time graphing of signals from auxiliary inputs and ion-selective electrodes providing scope for comprehensive analysis of oxygen activity simultaneously with signals such as pH, TPP+, calcium, potassium and hydrogen ions.

LED1 Light Source

An LED1 high-intensity light source in one of three colors is supplied with the  Chlorolab 2+ system . The different light sources that can be supplied are: LED1/R: red LED-peak wavelength 627nm LED1/B: blue LED-peak wavelength 470nm LED1/W: white LED with a color temperature of 4100K Light intensity adjustments are made automatically based on user-defined PFD tables within OxyTrace+ software. PFD tables consist of up to 20 individual steps allowing complex light response assays to be configured for automatic execution during a measurement.

Each of the LED1 light sources provide a maximum intensity of up to 2,000 µmol m -2   s -1  in DW2/2. Greater intensities are achievable by purchasing additional LED1 housings and an adapter which allows simultaneous automatic control of up to 3 LED1 light units.

Quantitherm PAR/Temp Sensor The Quantitherm consists of the QRT1 handheld display unit combined with the QTP1+ probe sensor. For use as a calibration tool for the  LS2 light source , the QTP1+ probe allows for real-time measurement of light intensity via the built-in screen. OxyTrace+ Software Automated 2-step calibration routine guides the user quickly and effectively through the system calibration process using electrode values measured from air-saturated and deoxygenated water. Simple configuration of comprehensive PFD tables consisting of up to 20 individual light steps. Light intensity adjustments are performed automatically during the measurement. Calibrate the light source from a simple software routine. Automatic calculation of oxygen rates from user-defined rate intervals. Automatically calculate rates of change for defined PFD light steps with a calculation of quantum yield presented at the end of a measurement.

Contact us to learn more about the Chlorolab 2+ : The liquid-phase oxygen electrode system for advanced photosynthesis & respiration studies

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