The iLite® FGF21 Assay Ready Cells can be used in an assay for the quantification of the potency of analogues of human FGF21, the potency of FGFR1c antagonists or a neutralizing antibody response against such products in human serum in the absence of serum matrix effects.
iLite® FGF21 Assay Ready Cells are based on the human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered and optimized to be responsive to human FGF21 and analogues of human FGF21 by specific and proportional expression of Firefly Luciferase.
Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
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Key benefits of iLite® FGF-21 Assay Ready Cells:
Unparalleled sensitivity (>10 fold inductions)
Highly specific reporter gene cell lines
Normalization read-out included
Negative control available for screening of unspecific activity
Easy to use - ready-to-use from the freezer, without culturing of cells
