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MRS Agar
Medium for isolation and enumeration of mesophilic lactic acid bacteria, according to ISO 15214

MRS Agar is a medium used for the cultivation of Lactobacillus spp from all types of materials including samples from oral and fecal sources. It may also support the growth of Pediococcus and Leuconostoc species as well as other secondary bacteria. This medium complies with the recommendations of ISO 15214 and APHA. 

Enzymatic digest of casein and meat extract provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Ammonium citrate and sodium acetate are the selective agents effective against streptococci and moulds. The low pH is also inhibitory for most organisms other than lactobacilli. Magnesium and manganese sulfates are sources of ions and sulfate acting as growth stimulants. Dipotassium phosphate is the buffer. Glucose is the fermentable carbohydrate. Tween 80 is an emulsifier. Agar is the solidifying agent.


6 bottles x 200 mL
ref. 412320

storage temperature 10-25 °C
2 years shelf life

M-Green Yeast and Mold Agar pH modified
Selective medium for detection and enumeration of fungi in beverages

Peptones, yeast extract and dextrose provide nutrients for growth. Magnesium sulphate and potassium phosphate are the buffering agents. Diastase is a mixture of enzymes which hydrolyze starch. Bromocresol green is a pH indicator. The low pH inhibits the bacterial contamination. Agar is the solidifying agent.

Filter the sample through a sterile membrane and transfer the membrane on the agar surface. Then, cover the plate with the lid, turn it upside down and incubate at 30-35°C for 48 hours and up to 5 days in an aerobic atmosphere with increased humidity.

After incubation, colonies appearing on the filter surface can be counted. Mold colonies appear green and filamentous, whereas yeast colonies are green and opaque. 


20 plates 60 mm
4 sleeves, 5 plates each
ref. 163842L

storage temperature 10-25 °C
180 days shelf life

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