Leaflab 2+ : The gas-phase oxygen electrode system for advanced photosynthesis & respiration measurements

Ideal for high level lab research as well as teaching environments.

The Leaflab 2+ gas-phase oxygen electrode system facilitates advanced studies of photosynthesis and respiration from solid-state samples. Ideally suited to high-level lab research, it is equally at home in teaching environments for under and post-grad plant biology studies of the photosynthetic processes. Samples typically consist of leafdiscs which are either cut from a broad leaf or made up of a “mat” of smaller leaves to form a circular disc of 10cm 2 . Changes in oxygen content of the sealed sample chamber are determined by the integral oxygen electrode mounted in the base of the chamber. Leaflab 2+ Highlights:

PC-operated USB Oxylab+ electrode control unit LD2/3 gas-phase electrode chamber with dual water jacket for effective sample/sensor temperature control LH36/2R LED light source (up to 750 µmol m-2 s-1) with automated control via user-defined PFD light tables Suitable for gas-phase measurements from leafdisc samples of 10cm2 24-bit high-resolution measurement of oxygen signals. Integral systems for measurement of auxiliary signals with 16-bit resolution Onboard LCD readings of oxygen & auxiliary signals 2-channel capability via purchase of additional systems QSRED sensor for light source calibration OxyTrace+ Windows® software for data acquisition, analysis & hardware control Real-time 0V – 4.5V analogue output of oxygen signal

Leaflab 2+ System Components:

S1 Oxygen Electrode Disc When connected to the electrode control unit, the S1 provides a fast, effective and accurate method of detecting small changes in oxygen concentration. LD2/3 Electrode Chamber The LD2/3 leafdisc electrode chamber allows oxygen uptake/evolution measurements to be performed from leafdiscs, excised needles, algae, mosses, lichens etc with a surface area of up to 10cm 2

Oxylab+ Control Unit

Oxylab+ provides a convenient yet powerful tool for measurements of oxygen evolution or uptake across a broad range of liquid-phase samples from chloroplast extractions to mitochondrial suspensions with oxygen concentrations up to 100%. 24-bit resolution allows detection of minute changes in oxygen tension without needing to apply instrument gain, resulting in noise-free traces even when zoomed in on areas of interest.

The system allows real-time graphing providing scope for comprehensive analysis of oxygen activity

LH36/2R LED Light Source The large-area LED array consists of 36 red LEDs arranged in such a way to provide a high uniformity of illumination of laminar samples suspended in the square section chamber of the DW3. The LH36/2R has a peak wavelength centered at 660nm with a maximum intensity of 900 µmol m -2 s -1 . QSRED Quantum Sensor QSRED assists with effective calibration of the LH36/2R light source. Each unit is supplied with an individual certificate showing that system calibration has been carried out against a National Physical Laboratory (NPL) calibrated reference lamp.

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Data Sheet

PLANT BIOLOGY 2020 JULY 27 - 31

ESA 2020 AUGUST 2 - 7

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