1.  To establish a correlation between ULV and bottle bioassay results.
2.  To generate updated resistance profiles for the CA mosquito vectors. 
3.  To provide districts with the most recent insecticide resistance data so they can make the best decisions for their control programs.

1. Collect wild mosquitoes from a specific location to use for testing. We are most interested in Cx. pipiens/quinqs., Cx. tarsalis, and Ae. aegypti, as they are currently the major disease risk vectors in California.

• For Cx. pipeins/quinq., gravid females are preferred. 
• Cx. tarsalis is more difficult, so we will take adults or larvae (whichever you can get in the highest numbers). We often have to run the tests on F1s.
• For Ae. aegypti, collected eggs are preferred, but adult females are also an option.
• Note: A standard bottle bioassay requires 120 adult females from a single species per insecticide tested (x2 if using a synergist such as PBO). So obviously, the more specimens you collect for us, the easier it will be.
• Note: Collected mosquitoes should be from a specific location. Using multiple trapping sites is okay as long as they are relatively close to each other. Be sure to record the exact location(s) where the specimens were collected.

1. Provide us with a list of adulticides that you want the mosquitoes to be tested against. It is recommended that you list the adulticides you use most often in your control programs. We are most interested in the pyrethroids Pyrethrum +/- PBO and Deltamethrin and the organophosphates Naled and Malathion, but we can run other adulticides at your request.
2.  Arrange transport of the mosquito specimens to our lab in Parlier, CA for testing. Often this will require someone from our lab to drive to your facility for pick-up.