The GeneNav™ HPV Complete qPCR Kit uses probe-based qPCR technology to detect HPV with high specificity. Each sequence-specific probe contains a 5' fluorophore and a 3' quencher. On its own, the probe's 3' quencher effectively absorbs the emission from the nearby 5' fluorophore so that no net fluorescence is detected. During PCR amplifcation, the probe binds to its specific target template. While replicating the DNA sample, the PCR Taq Polymerase's 5' to 3' exonuclease activity also hydrolyzes the probe, releasing a free-floating 5' fluorophore that is detectable by the qPCR machine and can be translated into an amplification plot. This HPV Complete qPCR kit identifes 14 types of high risk HPV, 2 types of low risk HPV, and a human ACTIN control using 4 fluorescent channels in one tube: ROX, FAM, HEX and Cy5. As a result, this kit can not only detect the 14 high risk HPV types and 2 low risk HPV types, it is also capable of distinguishing HPV 16 and 18 from all the other types. The internal control, human ACTIN, indicates the quality of extracted DNA.

Purpose

Continuously monitoring the HPV infection status of your patient, to ensure that the
infection of one specific genotype does not persist longer than two years; it is the
persistence of one HPV genotype, rather than infection itself, that leads to cervical cancer.