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2-AB-labeled Fetuin N-linked Sialylated Glycan Separation
Comparison - Proteomix SAX -
3 µm, 4.6 x 50 mm vs. 5 µm, 2.1 x 250 mm
Column 1: Proteomix SAX-NP3, 3 µm, 4.6 x 50 mm (PN: 403NP3-4605), UPLC, 430 bar, Flow Rate: 1 mL/min;
Column 2: Proteomix SAX-NP5, 5 µm, 2.1 x 250 mm PEEK (PN: 403NP5P-2125), HPLC, 225 bar, Flow Rate: 0.2 mL/min
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Mobile phase: A: H2O + 20% ACN, B: 250 mM Ammonium Formate pH 4.5 + 20% ACN
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Detector: Excitation 260 nm, Emission 430 nm; PMT 10
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Column temperature: RT;
Injection volume: 3.0 µL and 1.5 µL;
Sample
: N-Linked Glycans Bovine Fetuin
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