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Small Molecule Drug Application:
Irinotecan Analysis
Opalshell™-C18 vs Conventional 5 µm C18
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Natural Product Application:
Ginseng Saponins Extract Analysis
Competition Comparison
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Column
: Opalshell™-C18 (2.6 µm, 4.6 x 50 mm), Conventional
C18 ( 5 µm, 4.6 x 250 mm)
;
Mobile Phase
: A : 2.72 g/L KH2PO4, pH 3.5 by 1/20 H3PO4, B: ACN : MeOH = 3 : 2;
Injection
:
10 µL;
Flow Rate
: 0.6 mL/min;
Detection
: UV 220 nm;
Temperature
: Ambient;
Sample
: Irinotecan sample mixture
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Column: Opalshell™-C18 (2.6 µm, 4.6 x 50 mm), Agilent Poroshell 120 C18 (2.7µm, 4.6 x 50 mm);
Mobile Phase:
A: ACN; B: H2O;
Injection
: 10 µL;
Flow Rate
: 1.5 mL/min;
Detection
: UV 203 nm;
Temperature
: Ambient;
Sample
: 2.5 mg/mL diluted by 70% methanol
*Disclaimer: Agilent and Poroshell are registered trademarks of Agilent Technologies; Comparative separations may not be representative of all applications.
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