MAb IgG1 and IgG2 Charge Variants Separation by Narrow-bore SCX Column

Sepax Technologies, Inc.

High Resolution MAb IgG1 and IgG2 Charge Variants Separation

by Sepax Proteomix® Narrow-bore (2.1mm I.D.) SCX column

Sepax Proteomix® Narrow-bore (2.1 mm I.D.) SCX column offers:

Ultra-High Resolution
High Sensitivity
Small sample amount requirement
Reduced solvent consumption

Experiment

Vectibix (PanitumumAb) IgG2 Charge Variants Separation - pH Gradient

Sample:

IgG2 Vectibix (PanitumumAb)

IgG1 Erbitux (Cetuximab)

IgG1 Rituxan (Rituximab)

Column:

Proteomix SCX NP-5, 5 um, 2.1 x 250 mm PEEK

(PN: 401NP5P-2125)

Flow Rate: 0.3 mL/min;

Detection: UV 280 nm; Temperature: 25°C;

Download App Note: PM1019

Mobile Phase A: 16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCL pH 5.6 Mobile Phase B : 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCL pH 10.2 ; Sample : Vectibix 10 mg/mL diluted with water from 20 mg/mL stock; Injection Volume : 1 µL

Erbitux (Cetuximab) IgG1 Charge Variants Separation - pH Gradient

Rituxan (Rituximab) IgG1 Charge Variants Separation - Salt Gradient vs. pH Gradient

Mobile Phase A: 16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCL pH 5.6; Mobile Phase B: 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCL pH 10.2 Mobile Phase C: B+1 M NaCl, Sample: 2 mg/mL Erbitux; Injection Volume: 3 µL

Salt Gradient: Mobile Phase A:10 mM Sodium Phosphate pH 7.5; Mobile Phase B: A + 1M NaCl; pH Gradient: Mobile phase A: 16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCl, pH 5.6; Mobile phase B: 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCl, pH 10.2; Mobile phase C: B+1 M NaCl; Sample : Rituximab 10 mg/mL; Injection Volume : 1 µL