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HAPPY HOLIDAYS from our SEC Family to Yours
Analytical and Preparative Size Exclusion Chromatography
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Unix™, Zenix®/ Zenix®-C, SRT®/ SRT®-C, and SRT®-10/ SRT®-10C are seven SEC product lines developed based on innovative surface coatings and particle technologies comprised of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on 1.8 µm, 3 µm, 5 µm, or 10 µm high purity and mechanically stabilized silica for UHPLC, HPLC, and FPLC use of Analytical and Preparative Size Exclusion applications.
Utilizing two unique surface coating technologies - stand-up monolayer for Unix™, Zenix®, SRT® and SRT®-10; and lay-down monolayer for Zenix®-C, SRT®-C, and SRT®-10C, on porous silica, Sepax offers optimized surface chemistry choices based on sample type and sample hydrophobicity in order to achieve high resolution performance separation.
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Find the Suitable Pore Size
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Overlays of Protein Separation on Different Pore Sizes of Zenix 3
µm SEC Columns
Column: Zenix-SEC (3 µm, 80, 100, 150, 300 Å, 7.8 x 300 mm each);
Mobile phase: 150 mM Phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min;
Detector: UV 214 nm;
Column temperature: 25 C;
Injection volume: 5 µL
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Overlays of Protein Separation on Different Pore Sizes of SRT-C 5
µm SEC Columns
Column: SRT-C -SEC (5 µm, 300, 500, 1000, 2000 Å, 7.8 x 300 mm each);
Mobile phase: 150 mM Phosphate buffer, pH 7.0;
Flow rate: 1.0 mL/min;
Detector: UV 214 nm;
Column temperature: 25 C ;
Injection volume: 5 µL
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Choose the Suitable SEC Surface Chemistry
Sepax offers surface chemistry choices based on sample type and sample hydrophobicity in order to achieve high resolution performance separation. Unix™, Zenix®, SRT® and SRT®-10 use stand-up monolayer bonded on porous silica. Zenix®-C, SRT®-C, and SRT®-10C use lay-down monolayer on porous silica.
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Separation of Protein Standards on
Zenix and Zenix-C SEC-300
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| Column: Zenix SEC-300, 3µm, 100Å, 7.8×300 mm and Zenix-C SEC-300 3µm, 100Å, 7.8×300 mm; Mobile Phase: 150mM phosphate buffer, pH 7.0; Flow rate: 1.0 mL/min; Injection Volume: 20 µL; Detector: UV 280 nm; Sample: Proteins thyroglobulin, BSA, Ribonuclease A (2 mg/mL) |
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Separation of Hydrophobic Monoclonal Antibody F on Zenix and Zenix-C SEC-300
| Column: Zenix SEC-300, 3µm, 100Å, 7.8×300 mm and Zenix-C SEC-300 3µm, 100Å, 7.8×300 mm; Mobile Phase: 150mM phosphate buffer, pH 7.0; Flow rate: 1.0 mL/min; Detection: UV 280 nm; Sample: mAb F 1.23 mg/mL in 10mM sodium succinte, pH 5.0 |
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Unsure which column is best for your work?
- From our Family to yours, we wish you a safe and wonderful holiday season with our gift to you
EOY16-ST729 (Good until 12/31/2016)
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www.sepax-tech.com · 1-877-SEPAX-US
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