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SEPAX SEC-MALS Columns and Services
SRT/SRT-C 1000Å and 2000Å SEC-MALS Columns for Large Molecules
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Large Pore SEC Light Scattering
Certified
Columns
Sepax
SRT
and
SRT-C
1000 Å and 2000 Å large pore light scattering SEC columns provide excellent analytical characterization of large molecules including:
Viruses,
Virus like particles (VLPs), Nanoparticles, and
Protein complexes.
Together with UV, MALS, RI triple detectors, Sepax Large Pore SEC-LS certified columns, can provide valuable information on molecular weight, polydispersity (Mw/Mn), and radius of gyration (Rg) for components such as aggregates, monomers and fragments.
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Light Scattering Service
Need to characterize your samples by light scattering? Sepax Light Scattering Service offers the exciting capability of determining the native molecular weights of proteins, their protein:protein and other macro molecular complexes. Our experts use this technique with advanced MALS detectors for high sensitivity, reliability, and signal quality measurements from multi-angle light scattering systems.
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Showcase: VLP Screening on Sepax SEC-MALS Columns
- Two different VLPs
- Pore sizes: 1000 Å and 2000 Å
- SEC surface chemistries: SRT and SRT-C
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VLP #1 SEC-MALS Analysis on SRT 1000 Å LS SEC Column
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Column: SRT SEC-1000 (5 um, 1000 Å), 7.8x 300 mm, Light Scattering Column;
Mobile phase: 25 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH 7.5; Flow rate: 1 mL/min; Detector: UV 280 nm, RI, MALS;
Column temperature: 25 C;
Injection volume: 50 m
g protein VLP # 1
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VLP #2 SEC-MALS Analysis on SRT 1000 Å LS SEC Column
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Column: SRT SEC-1000 (5 um, 1000 Å), 7.8x 300 mm, Light Scattering Column; Mobile phase: 25 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH 7.5; Flow rate: 1 mL/min; Detector: UV 280 nm, RI, MALS;
Column temperature: 25 C;
Injection volume: 50 mg protein VLP # 2
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VLP #2 SEC-MALS Analysis on SRT-C 2000 Å LS SEC Column
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Column: SRT-C SEC-2000 (5 um, 2000 Å), 7.8x 300 mm, Light Scattering Column; Mobile phase: 25 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH 7.5; Flow rate: 1 mL/min; Detector: UV 280 nm, RI, MALS;
Column temperature: 25 C;
Injection volume: 50 mg protein VLP # 2
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VLP #2 SRT SEC-1000 vs. SRT-C SEC-2000
Column: SRT SEC-1000(5 um, 1000 Å),SRT-C SEC-2000 (5 um, 2000 Å), 7.8x 300 mm, Light Scattering Columns; Mobile phase: 25 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH 7.5; Flow rate: 1 mL/min; Detector: UV 280 nm, RI, MALS; Column temperature: 25 C; Injection volume: 50 mg protein VLP # 2
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www.sepax-tech.com · 1-877-SEPAX-US
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