Track record of Literature References 
                            on Sepax SEC columns and methods 

 

Neeraj Kohli, Nidhi Jain, Melissa L. Geddie, Maja Razlog, Lihui Xu & Alexey A. Lugovskoy
Mabs online, DOI:10.1080/19420862.2015.1048410, published May 11, 2015
Shatz W, Chung S, Li B, Marshall B, Tejada M, Phung W, Sandoval W, Kelley B, Scheer JM
Mabs, 5:6, 2013, 872-881
Martin L, Anne S, Alexandra P, Cristina G, Michael L, Philippe D
Journal of Immunological Methods, v393, Issues 1-2, 31 July 2013, Pages 81-85

Kemin T, Gekleng Chhor, T. Andrew B, Robert P. Jedrzejczak, Magdalena M and Andrzej J
The Journal of biological chemistry, 2014 May 2; 289(18): 12232-44

Beata Jastrzebska, Philipe Ringler, Krzysztof Palczewski, Andreas Engel
Journal of Structural Biology, 6 March 2013

Application Highlights:
 
* Track record of Literature References on Sepax SEC columns and method.

* Sepax SEC columns can be successfully applied to biological sample separations such as MAb, BsMab, fusion proteins, membrane proteins and more.

* Sepax SEC columns are suitable for protein separations with a variety of detergents as additives in the mobile phase, and with a selection of different pore sizes to accommodate a wide range of separations for different protein molecular weights.

Herceptin Analysis on Zenix SEC-300 

Column: Zenix SEC-300 (3 µm, 300 Å, 7.8 x 300 mm)

Mobile phase: 150 mM phosphate buffer; 

Flow rate: 1mL/min; Detector: UV 280 nm;

Column temperature: 25°C; Injection volume: 10 µL; Samples: Herceptin 2.34 mg/mL
IgM from Mouse Ascites Analysis on SRT
Column:  SRT SEC-500  (5 µm, 500 Å, 7.8 x 300 mm)

Mobile phase: 150 mM sodium phosphate buffer, pH 7.0

Flow rate: 1 mL/min; Detector: UV 280 nm;

Column temperature:25°C; Injection: 50 mL mouse ascitic fluid (1mg/mL IgM)